Using genetic engineering bacteria
Bacteria are tools useful for genetic engineering modification. Through the use of plasmids (circular bacterial DNA exchangeable portions) other organisms such as bacteria can be genetically transformed. This guide describes the basic methods that apply to the use of genetically engineered bacteria in another organism.
Transform other species of bacteria
Obtain a sample of the first species of bacteria sweeping the colony with a sterile loop.
Shake the loop inoculated in a test tube through a water bath at a temperature of about 48 degrees Celsius for five seconds .
Add a couple of millimeters restrictor enzyme to the test tube and inoculated with heated loop. The restrictor enzyme varies with the gene of interest to be transferred to other species of bacteria.
Dip a second sterile loop into a colony of the second species of bacteria, which will transform.
Bacteria Spread evenly on a Petri plate containing agarose medium, inoculated loop sliding through agarose.
Allow to incubate bacteria two days in the incubator, so that bacterial colonies to form.
Add the mixture created above containing the gene of interest to the petri dish containing bacterial colonies.
Let the bacteria incubate for two days in the incubator. This allows the kind adopt added to the plate and insert genes in its genome.
Design a method to test the transformed bacteria to test whether genes have assimilated. For example, if the added gene confers resistance to antibiotics bacteria can be practical to add disks ampicillin agar plates. If they survive, have adopted the gene.